Application of DNA marker technologies in characterizing genome diversity of selected coffee varieties and accessions from India
Assessment of genome diversity in a gene pool of any particular species offers great promise for better exploitation and management of genetic resources. The development of molecular markers in coffee (Coffea spp.) trees has opened new perspectives for analysing genetic relationships and in marker assisted breeding. In this study, we report the successful use of two efficient PCR-based DNA marker technologies, amplified fragment length polymorphism (AFLPs) and simple sequence repeats (SSRs) for generating DNA profiles of the selected C. arabica accessions under cultivation and gene bank collections of C. canephora available in India. Eighteen Arabica genotypes covering the commercial accessions S.288, S.795 and S.1934 were subjected to AFLP analysisusing 36 different primer combinations. Analysis of the DNA fingerprints revealed 18-45 amplified products depending on the genotype and primer set. A total of 115 polymorphic bands were scored. The high polymorphism was mainly attributed to the introgression of C. liberica genetic material as these commercial lines were derived from S.26, a putative natural interspecific hybrid (C. arabica x C. liberica) and thus the molecular data established the natural hybrid lineage. Further, 39 accessions of C. canephora representing indigenous as well as exotic gene bank collections were analysed for genetic diversity in comparison with 16 IRD collections from different geographical regions of the world, using 15 AFLP primer combinations and 12 SSR primer sets. A total of 205 polymorphic bands were scored from the AFLP marker data while 72 alleles were identified with 12 SSR primers. Analysis of data for genetic relationships revealed the structure of Indian germplasm and the outcome is almost similar with both marker approaches. The potential use of AFLPs and SSRs for evaluating genetic diversity and in other molecular genetic applications are discussed.
| Main Authors: | , , , , , , |
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| Format: | biblioteca |
| Published: |
Trieste (Italia) ASIC
2001
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| Subjects: | COFFEA ARABICA, COFFEA CANEPHORA, COFFEA LIBERICA, GENES, CAFE, ADN, BANCO DE GENES, MARCADORES GENETICOS, POLIMORFISMO GENETICO, GENOMAS, GENOTIPOS, HIBRIDACION INTERESPECIFICA, INTROGRESION, GENETICA MOLECULAR, PCR, INDIA, |
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