Genetic diversity and introgression analyses in coffee (Coffea arabica L.) using molecular markers

DNA markers (AFLP, RAPD, RFLP, SSR) were recently used to assess the genetic diversity among wild and cultivated C. arabica accessions, and to detect introgressions from C. canephora and C. liberica into C. arabica genome. The results allowed for the definition of breeding strategies using the whole genetic diversity that are conserved in field gene banks and for the control of alien gene transfer to improve arabica cultivars. Almost all polymorphism was generated by the Ethiopian material. The southwestern Ethiopian accessions were grouped separately from the southeastern Ethiopian accessions. The cultivars were classified according to their genetic origin (i.e. Typica or Bourbon). The Yemen cultivars were grouped with the Typica-derived accessions, confirming the Yemen origin of the coffee plant cultivated in Amsterdam and Paris at the beginning of the 18th century and later known as Typica. Introgressions of C. canephora and C. liberica were identified in derivatives from natural interspecific hybrids (i.e. Timor Hybrid and S.26). The introgressed genotypes were distinguished from the C. arabica accessions by additional bands (i.e. introgressed markers) and missing bands (i.e. markers related to introgression process). The missing bands might be associated with the stabilization process of introgressed fragments over the generations. Segregation of the C. canephora genome in the tetraploid interspecific hybrid (C. arabica x C. canephora) was studied using a complete linkage map of C. canephora. The chromosomes segregated at random in the tetraploid hybrid, indicating the absence of preferential pairing of the four sets of chromosomes. Recombination in the tetraploid hybrid was not significantly restricted by the genetic differentiation of chromosomes belonging to the different genomes.

Bibliographic Details

Main Authors:	43095 Anthony, F., 58486 Combes, M.C., 77694 Herrera, J.C., 106746 Prakash, N.S., 48644 Bertrand, B., 85180 Lashermes, P., 3180 Association Scientifique Internationale du Cafe, París (Francia), 32308 19. International Scientific Colloquium on Coffee Trieste (Italia) 14-18 May 2001
Format:	biblioteca
Published:	Trieste (Italia) ASIC 2001
Subjects:	COFFEA ARABICA, COFFEA CANEPHORA, COFFEA LIBERICA, CROMOSOMAS, MAPAS GENETICOS, MARCADORES GENETICOS, VARIACION GENETICA, HIBRIDOS, INTROGRESION, GENETICA MOLECULAR, RAPD, RFLP, SEGREGACION, TETRAPLOIDIA,
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