Loop-mediated isothermal amplification (LAMP) assay for the diagnosis of fasciolosis in sheep and its application under field conditions

[Background] Loop-mediated isothermal amplification (LAMP) is a very specific, efficient, and rapid gene amplification procedure in which the reaction can run at a constant temperature. In the current study we have developed a LAMP assay to improve the diagnosis of Fasciola spp. in the faeces of sheep.

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Bibliographic Details
Main Authors: Martínez Valladares, María, Rojo Vázquez, Francisco Antonio
Other Authors: CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
Format: artículo biblioteca
Language:English
Published: BioMed Central 2016-02-05
Subjects:Fasciola spp, Sheep, Diagnosis, PCR, Loop-mediated isothermal amplification (LAMP),
Online Access:http://hdl.handle.net/10261/143361
http://dx.doi.org/10.13039/100007652
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100000780
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Summary:[Background] Loop-mediated isothermal amplification (LAMP) is a very specific, efficient, and rapid gene amplification procedure in which the reaction can run at a constant temperature. In the current study we have developed a LAMP assay to improve the diagnosis of Fasciola spp. in the faeces of sheep.