Optimization of thin film-microextraction (TF-SPME) method in order to determine musts volatile compounds
It is well known that grape aromatic composition is directly correlated to the final wine quality. To determine this composition, a previous stage of selective extraction is necessary, since the aromatic compounds are found in very low concentrations in the grapes. Therefore, in this work, the thin film microextraction technique (TF-SPME) was optimized, for first time, with the aim to analyze the volatile composition of the grape musts. The results obtained with the two commercially available absorbent materials for TF-SPME, polydimethylsiloxane/carboxene (PDMS/CAR) and PDMS/divinylbenzene (PDMS/DVB), were optimized and compared. To carry out the optimization, a randomized factorial design was performed combining the following factors and levels: extraction mode (headspace (HS), or direct immersion (DI)), stirring speed (500 and 1000 rpm), extraction time (1, 3 and 6 h), and extraction temperature (20, 40 and 60 °C). After performing a principal component analysis (PCA) and an analysis of variance (ANOVA) multifactorial, it was concluded that the best conditions for TF-SPME with PDMS/CAR were: direct immersion (DI), 500 rpm, 6 h, and 20 °C, while for TF-SPME with PDMS/DVB no conditions were found that maximized the extraction of most compounds, therefore compromise conditions were chosen: headspace (HS), 500 rpm, 6 h, and 40 °C. Finally, the comparison between the results obtained with both absorbents indicated that the absorbent that extracted better the volatile compounds from the musts with the TF-SPME technique, was PDMS/CAR, under the conditions: direct immersion (DI), 500 rpm, 6 h, and 20 °C.
| Main Authors: | , , , , , |
|---|---|
| Other Authors: | |
| Format: | artículo biblioteca |
| Published: |
Elsevier BV
2022-09-15
|
| Subjects: | TF-SPME, Optimization, Extraction, Comparison, Grape aroma, PDMS/CAR and PDMS/DVB absorbents, |
| Online Access: | http://hdl.handle.net/10261/287747 http://dx.doi.org/10.13039/501100011033 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | It is well known that grape aromatic composition is directly correlated to the final wine quality. To determine this composition, a previous stage of selective extraction is necessary, since the aromatic compounds are found in very low concentrations in the grapes. Therefore, in this work, the thin film microextraction technique (TF-SPME) was optimized, for first time, with the aim to analyze the volatile composition of the grape musts. The results obtained with the two commercially available absorbent materials for TF-SPME, polydimethylsiloxane/carboxene (PDMS/CAR) and PDMS/divinylbenzene (PDMS/DVB), were optimized and compared. To carry out the optimization, a randomized factorial design was performed combining the following factors and levels: extraction mode (headspace (HS), or direct immersion (DI)), stirring speed (500 and 1000 rpm), extraction time (1, 3 and 6 h), and extraction temperature (20, 40 and 60 °C). After performing a principal component analysis (PCA) and an analysis of variance (ANOVA) multifactorial, it was concluded that the best conditions for TF-SPME with PDMS/CAR were: direct immersion (DI), 500 rpm, 6 h, and 20 °C, while for TF-SPME with PDMS/DVB no conditions were found that maximized the extraction of most compounds, therefore compromise conditions were chosen: headspace (HS), 500 rpm, 6 h, and 40 °C. Finally, the comparison between the results obtained with both absorbents indicated that the absorbent that extracted better the volatile compounds from the musts with the TF-SPME technique, was PDMS/CAR, under the conditions: direct immersion (DI), 500 rpm, 6 h, and 20 °C. |
|---|