Antigenic extinction of the v-319 acatlan modified live virus rabies vaccine in sheeps
In order to obtain the required titer for the V-319 Acatlan live virus vaccine necessary to protect sheep against rabies in endemic areas, five Rambouillet cross bred lambs per group were vaccinated in accordance with the following viral titers: Group A 104.0, Group B 105.1 and Group C 106.19 LD50 per dose. Five lambs (Group D) were not vaccinated and used as negative control. Blood samples were taken at 0, 15, 30, 60, 90, 180 and 360 d post vaccination. Antibody titers were obtained by means of a serum neutralization test. Nine rabies negative lambs were used as positive control for the CASS-88 challenge strain of vampire origin with a 7.7 x 106.0 LD80 in mice titer. Results show that the modified V-319 Acatlán live virus vaccine produced good protection to Group C up to 360 d post vaccination, with an average 1.10 IU protection at 15 d, 1.42 IU at 30 d, 5.28 IU at 60 d, 3.87 IU at 90 d, 2.0 IU at 180 d and 1.10 IU at 360 d. In Groups A and B antibodies were found only at 90 and 180 d post vaccination so they weren',t challenged. Four of the Group C lambs survived the challenge (80 %) and 88.9 % (9 animals) of the positive control group died. Group D was negative. These results prove that the V-319 Acatlán live virus rabies vaccine with a 106.19 LD50 virus titer per dose meets all the requirements to protect sheep for 360 d as required by the Mexican Official Standards (NOM-35-ZOO-1996).
| Auteurs principaux: | , , , |
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| Format: | Digital revista |
| Langue: | spa |
| Publié: |
Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias
2012
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| Accès en ligne: | https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/1275 |
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