Optimization of lactic acid production by Lactobacillus plantarum strain Hui1 in a medium containing sugar cane molasses
The aim of this study was to optimize lactic acid production by a native strain (Hui1) of Lactobacillus plantarum isolated from a Peruvian Amazon fruit (Genipa americana) in a medium supplemented with an agroindustrial by-product such as sugar cane molasses. Optimization was performed though one-factor-at-a-time studies followed by the Placket-Burman and central composite designs. The data were analyzed by using the Statistica® 10 software. Several carbon, nitrogen and ion sources were tested, and the optimum concentration of lactic acid achieved was 84.2 g L-1 in a medium containing as follows (in g L-1): meat extract, 18.69; tryptone, 7.88; sugar cane molasses, 140; calcium carbonate, 15; dipotassium phosphate, 1; manganese phosphate, 0.03; sodium acetate, 5, and magnesium sulphate, 0.2. In addition, a high degree of conversion from sugar cane molasses to lactic acid was obtained (Yp/s 0.898 g g-1). These results indicate the potential of Lactobacillus plantarum strain Hui1 to produce lactic acid in a medium supplemented with sugar cane molasses, an underutilized industrial by-product.
| Principais autores: | , , , , , |
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| Formato: | Digital revista |
| Idioma: | eng |
| Publicado em: |
Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias Agrarias
2021
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| Acesso em linha: | https://revistas.unal.edu.co/index.php/agrocol/article/view/89674 |
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