IN VITRO BIOCONTROL ACTIVITY OF MACROFUNGI AGAINST DIFFERENT PLANT PATHOGENIC FUNGI

IN VITRO BIOCONTROL ACTIVITY OF MACROFUNGI AGAINST DIFFERENT PLANT PATHOGENIC FUNGI

Fungal diseases in plants are a major concern in agricultural production. Macrofungi are a potential source of antifungal compounds that could be used to control these diseases. The aim of this study was to evaluate the in vitro biocontrol activity of four macrofungi of the genus Xylaria, Agrocybe, Psilocybe and Stereum on different plant pathogenic fungi. For this purpose, the growth curve of macrofungi was determined on two carbon sources: potato dextrose and wheat bran, interactions and the in vitro inhibition on phytopathogenic fungi were also characterized. Subsequently, extraction and characterization of secondary metabolites of the intracellular, extracellular and filtrate of homogenized of mycelia and broth culture were performed only on the fungi that showed greater inhibition percentage. Finally, the in vitro antifungal activity of this extracts was evaluated. Growth curves changes with the carbon source, three of four isolates show greater accumulation of biomass in potato dextrose than wheat brand reaching the stationary growth phase longer. Xylaria interactions were classified mostly as replacement, obtaining the highest level of antagonism on potato dextrose. Two of the three extracts assessed showed antifungal activity in all three pathogens isolated at concentrations of 18 µg/ml for biomass methanol extracts and 2.5 % for filtrate of homogenized with inhibitions from 10 % to 80 %. Characterization of the metabolites of Xylariashowed as possible compounds responsible for the activity of the fatty acids. This work showed the potential of these fungi for the control of fungal diseases. 

Saved in:
Bibliographic Details
Main Authors: Patiño Moscoso, Manuel Alfonso, Nieto Ramírez, Ivonne Jeannette, Chegwin Angarita, Carolina, Torres Rojas, Esperanza
Format: Digital revista
Language:spa
Published: Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Biología 2020
Online Access:https://revistas.unal.edu.co/index.php/actabiol/article/view/75303
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:www.revistas.unal.edu.co:article-75303
record_format ojs
institution UNAL
collection OJS
country Colombia
countrycode CO
component Revista
access En linea
databasecode rev-actabol
tag revista
region America del Sur
libraryname Sistema Nacional de Bibliotecas de la UNAL
language spa
format Digital
author Patiño Moscoso, Manuel Alfonso
Nieto Ramírez, Ivonne Jeannette
Chegwin Angarita, Carolina
Torres Rojas, Esperanza
spellingShingle Patiño Moscoso, Manuel Alfonso
Nieto Ramírez, Ivonne Jeannette
Chegwin Angarita, Carolina
Torres Rojas, Esperanza
IN VITRO BIOCONTROL ACTIVITY OF MACROFUNGI AGAINST DIFFERENT PLANT PATHOGENIC FUNGI
author_facet Patiño Moscoso, Manuel Alfonso
Nieto Ramírez, Ivonne Jeannette
Chegwin Angarita, Carolina
Torres Rojas, Esperanza
author_sort Patiño Moscoso, Manuel Alfonso
title IN VITRO BIOCONTROL ACTIVITY OF MACROFUNGI AGAINST DIFFERENT PLANT PATHOGENIC FUNGI
title_short IN VITRO BIOCONTROL ACTIVITY OF MACROFUNGI AGAINST DIFFERENT PLANT PATHOGENIC FUNGI
title_full IN VITRO BIOCONTROL ACTIVITY OF MACROFUNGI AGAINST DIFFERENT PLANT PATHOGENIC FUNGI
title_fullStr IN VITRO BIOCONTROL ACTIVITY OF MACROFUNGI AGAINST DIFFERENT PLANT PATHOGENIC FUNGI
title_full_unstemmed IN VITRO BIOCONTROL ACTIVITY OF MACROFUNGI AGAINST DIFFERENT PLANT PATHOGENIC FUNGI
title_sort in vitro biocontrol activity of macrofungi against different plant pathogenic fungi
description Fungal diseases in plants are a major concern in agricultural production. Macrofungi are a potential source of antifungal compounds that could be used to control these diseases. The aim of this study was to evaluate the in vitro biocontrol activity of four macrofungi of the genus Xylaria, Agrocybe, Psilocybe and Stereum on different plant pathogenic fungi. For this purpose, the growth curve of macrofungi was determined on two carbon sources: potato dextrose and wheat bran, interactions and the in vitro inhibition on phytopathogenic fungi were also characterized. Subsequently, extraction and characterization of secondary metabolites of the intracellular, extracellular and filtrate of homogenized of mycelia and broth culture were performed only on the fungi that showed greater inhibition percentage. Finally, the in vitro antifungal activity of this extracts was evaluated. Growth curves changes with the carbon source, three of four isolates show greater accumulation of biomass in potato dextrose than wheat brand reaching the stationary growth phase longer. Xylaria interactions were classified mostly as replacement, obtaining the highest level of antagonism on potato dextrose. Two of the three extracts assessed showed antifungal activity in all three pathogens isolated at concentrations of 18 µg/ml for biomass methanol extracts and 2.5 % for filtrate of homogenized with inhibitions from 10 % to 80 %. Characterization of the metabolites of Xylariashowed as possible compounds responsible for the activity of the fatty acids. This work showed the potential of these fungi for the control of fungal diseases. 
publisher Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Biología
publishDate 2020
url https://revistas.unal.edu.co/index.php/actabiol/article/view/75303
work_keys_str_mv AT patinomoscosomanuelalfonso invitrobiocontrolactivityofmacrofungiagainstdifferentplantpathogenicfungi
AT nietoramirezivonnejeannette invitrobiocontrolactivityofmacrofungiagainstdifferentplantpathogenicfungi
AT chegwinangaritacarolina invitrobiocontrolactivityofmacrofungiagainstdifferentplantpathogenicfungi
AT torresrojasesperanza invitrobiocontrolactivityofmacrofungiagainstdifferentplantpathogenicfungi
AT patinomoscosomanuelalfonso actividadbiocontroladorainvitrodemacrohongoscontradiferenteshongosfitopatogenos
AT nietoramirezivonnejeannette actividadbiocontroladorainvitrodemacrohongoscontradiferenteshongosfitopatogenos
AT chegwinangaritacarolina actividadbiocontroladorainvitrodemacrohongoscontradiferenteshongosfitopatogenos
AT torresrojasesperanza actividadbiocontroladorainvitrodemacrohongoscontradiferenteshongosfitopatogenos
AT patinomoscosomanuelalfonso atividadebiocontroladorainvitrodemacrohongoscontradiferentesfungosfitopatogenos
AT nietoramirezivonnejeannette atividadebiocontroladorainvitrodemacrohongoscontradiferentesfungosfitopatogenos
AT chegwinangaritacarolina atividadebiocontroladorainvitrodemacrohongoscontradiferentesfungosfitopatogenos
AT torresrojasesperanza atividadebiocontroladorainvitrodemacrohongoscontradiferentesfungosfitopatogenos
_version_ 1755914056811675648
spelling oai:www.revistas.unal.edu.co:article-753032020-05-06T22:10:40Z IN VITRO BIOCONTROL ACTIVITY OF MACROFUNGI AGAINST DIFFERENT PLANT PATHOGENIC FUNGI ACTIVIDAD BIOCONTROLADORA IN VITRO DE MACROHONGOS CONTRA DIFERENTES HONGOS FITOPATÓGENOS ATIVIDADE BIOCONTROLADORA IN VITRO DE MACROHONGOS CONTRA DIFERENTES FUNGOS FITOPATOGENOS Patiño Moscoso, Manuel Alfonso Nieto Ramírez, Ivonne Jeannette Chegwin Angarita, Carolina Torres Rojas, Esperanza Antagonism growth kinetics dual culture parasitism secondary metabolites Antagonismo cinética del crecimiento cultivo dual parasitismo metabolitos secundarios Antagonismo cinética de crescimento cultura dual parasitismo metabólitos secundários Fungal diseases in plants are a major concern in agricultural production. Macrofungi are a potential source of antifungal compounds that could be used to control these diseases. The aim of this study was to evaluate the in vitro biocontrol activity of four macrofungi of the genus Xylaria, Agrocybe, Psilocybe and Stereum on different plant pathogenic fungi. For this purpose, the growth curve of macrofungi was determined on two carbon sources: potato dextrose and wheat bran, interactions and the in vitro inhibition on phytopathogenic fungi were also characterized. Subsequently, extraction and characterization of secondary metabolites of the intracellular, extracellular and filtrate of homogenized of mycelia and broth culture were performed only on the fungi that showed greater inhibition percentage. Finally, the in vitro antifungal activity of this extracts was evaluated. Growth curves changes with the carbon source, three of four isolates show greater accumulation of biomass in potato dextrose than wheat brand reaching the stationary growth phase longer. Xylaria interactions were classified mostly as replacement, obtaining the highest level of antagonism on potato dextrose. Two of the three extracts assessed showed antifungal activity in all three pathogens isolated at concentrations of 18 µg/ml for biomass methanol extracts and 2.5 % for filtrate of homogenized with inhibitions from 10 % to 80 %. Characterization of the metabolites of Xylariashowed as possible compounds responsible for the activity of the fatty acids. This work showed the potential of these fungi for the control of fungal diseases.  Las enfermedades causadas por hongos en las plantas son una gran preocupación en la producción agrícola. Los macromicetos son una fuente potencial de compuestos antifúngicos que podrían usarse para controlar estas enfermedades. El objetivo de este estudio fue evaluar la actividad biocontroladora in vitro de cuatro macromicetos de los géneros Xylaria, Agrocybe, Psilocybe y Stereum sobre diferentes hongos fitopatógenos. Para ello, se determinó la curva de crecimiento de los macrohongos en dos medios: papa dextrosa (PDA) y salvado de trigo (ST) y se caracterizaron las interacciones y la inhibición in vitro de hongos fitopatógenos. Se realizó la extracción y caracterización de metabolitos secundarios de la biomasa, el medio extracelular y del homogeneizado del micelio y caldo en los hongos con mayor porcentaje de inhibición. Finalmente, se evaluó la actividad antifúngica in vitro de estos extractos. Las curvas de crecimiento cambiaron con la fuente de carbono, tres de cuatro macrohongos mostraron una mayor acumulación de biomasa en PDA que en ST. Las interacciones de Xylaria se clasificaron principalmente como reemplazo, obteniendo el mayor nivel de antagonismo en PDADos de los tres extractos evaluados mostraron actividad antifúngica contra los tres patógenos aislados en concentraciones de 18 μg/ml para extractos metanólicos de biomasa y 2,5 % para el filtrado de homogeneizado con inhibiciones de 10 % a 80 %.  La caracterización de los metabolitos de Xylaria mostró como posibles compuestos responsables de la actividad a los ácidos grasos. Este trabajo mostró el potencial de estos hongos para el control de enfermedades fúngicas. Doenças causadas por fungos em plantas são uma grande preocupação na produção agrícola. Os macromicetes são uma fonte potencial de compostos antifúngicos que poderiam ser usados para controlar essas doenças. O objectivo deste estudo foi avaliar a actividade de biocontrolo in vitro quatro macromycetes dos géneros Xylaria, Agrocybe, Psilocybe e Stereum em diferentes fungos patogénicos para plantas. Por isso, a curva de crescimento macrofungos duas meia determinado: de dextrose de batata (PDA) e farelo de trigo (ST) e interacções e inibição in vitro de fungos fitopatogénicos foram caracterizados. A extração e caracterização dos metabólitos secundários da biomassa, do meio extracelular e do homogeneizado do micélio e caldo nos fungos com maior porcentagem de inibição foram realizadas. Finalmente, a atividade antifúngica in vitro desses extratos foi avaliada. As curvas de crescimento alteradas com a fonte de carbono, três dos quatro macrófagos apresentaram maior acúmulo de biomassa no PCA do que no ST. interacções Xylaria são classificados principalmente como substituição, obtendo-se o maior nível de antagonismo dos três extractos PDADos avaliados apresentaram actividade antifúngica contra os três agentes patogénicos isolados em concentrações de 18 ug / ml de extractos metanólicos de biomassa e de 2,5% para filtrado homogeneizado com inibições de 10% a 80%.  A caracterização dos metabólitos de Xylaria mostrou-se como possíveis compostos responsáveis pela atividade dos ácidos graxos. Este trabalho mostrou o potencial desses fungos para o controle de doenças fúngicas. Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Biología 2020-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unal.edu.co/index.php/actabiol/article/view/75303 10.15446/abc.v25n2.75303 Acta Biológica Colombiana; Vol. 25 No. 2 (2020); 265 - 279 Acta Biológica Colombiana; Vol. 25 Núm. 2 (2020); 265 - 279 1900-1649 0120-548X spa https://revistas.unal.edu.co/index.php/actabiol/article/view/75303/pdf Derechos de autor 2020 Acta Biológica Colombiana https://creativecommons.org/licenses/by-nc-sa/4.0

Similar Items