IN VITRO BIOCONTROL ACTIVITY OF MACROFUNGI AGAINST DIFFERENT PLANT PATHOGENIC FUNGI
Fungal diseases in plants are a major concern in agricultural production. Macrofungi are a potential source of antifungal compounds that could be used to control these diseases. The aim of this study was to evaluate the in vitro biocontrol activity of four macrofungi of the genus Xylaria, Agrocybe, Psilocybe and Stereum on different plant pathogenic fungi. For this purpose, the growth curve of macrofungi was determined on two carbon sources: potato dextrose and wheat bran, interactions and the in vitro inhibition on phytopathogenic fungi were also characterized. Subsequently, extraction and characterization of secondary metabolites of the intracellular, extracellular and filtrate of homogenized of mycelia and broth culture were performed only on the fungi that showed greater inhibition percentage. Finally, the in vitro antifungal activity of this extracts was evaluated. Growth curves changes with the carbon source, three of four isolates show greater accumulation of biomass in potato dextrose than wheat brand reaching the stationary growth phase longer. Xylaria interactions were classified mostly as replacement, obtaining the highest level of antagonism on potato dextrose. Two of the three extracts assessed showed antifungal activity in all three pathogens isolated at concentrations of 18 µg/ml for biomass methanol extracts and 2.5 % for filtrate of homogenized with inhibitions from 10 % to 80 %. Characterization of the metabolites of Xylariashowed as possible compounds responsible for the activity of the fatty acids. This work showed the potential of these fungi for the control of fungal diseases.
| Main Authors: | , , , |
|---|---|
| Format: | Digital revista |
| Language: | spa |
| Published: |
Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Biología
2020
|
| Online Access: | https://revistas.unal.edu.co/index.php/actabiol/article/view/75303 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|