Design of expression cassettes using the Cry1Ba1 gene for potato (Solanum tuberosum L.) varieties
The most serious insect pest problem in the potato crop in Colombia is the lepidopteran Tecia solanivora that causessignificant economic losses. In this research, we designedexpression cassettes based on the cry1Ba1 gene of Bacillusthuringiensis that could confer resistance to T. solanivora via the variety Pastusa Suprema. We selected the elements of thedesigned expression cassettes through an analysis of scientificliterature and patent databases; the considered factors were theproteolytic activation of the Cry1Ba1 protoxin, modification ofcodonic use, polyadenylation signals, and cryptic splicing sites. We used a tissue-specific patatine promoter to reduce potentialbiosafety risks, because it is expressed only in the tuber. Thefreedom to operate analysis suggests that the commercial use of the designed expression cassettes in transgenic potato plants does not affect the rights of third parties in Colombia.
| Main Authors: | , , |
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| Format: | Digital revista |
| Language: | eng |
| Published: |
Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias Agrarias
2019
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| Online Access: | https://revistas.unal.edu.co/index.php/agrocol/article/view/70796 |
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