Evaluation of an in vitro system for the study of Spongospora subterranea f. sp. subterranea in potato roots (Solanum tuberosum subsp. andigena L.) Var. diacol capiro
The potato is the one of the main crops with global importance. According to the Colombian board of potato producers, the powdery scab is within the seven main phytosanitary problems of this crop in producing areas. The disease is caused by the pathogen Spongospora subterranea, which reduces the tubers yield and quality. The obligate nature of S. subterranea and the difficulty of isolating and maintaining strains have hampered the research on this disease. In this work we wanted to standardize the in vitro culture of potato roots infected with the pathogen, to allow its maintenance and proliferation, and subsequent research on the biology of the pathogen and in the interaction with their plants hosts. Here the plant roots were associated with the microorganism in an in vitro media under a controlled enviroment, whereas the aerial part developed photosynthesis. Evaluations conduced during two months after the roots were inoculated with disinfected cystosori showed that the duration of this culture system was conditioned by the contamination of the root media. However, in microscopic observations we were able to detect in potato roots the structures of Spongospora subterranea.
| Main Authors: | , , , , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Universidad Nacional de Colombia - Sede Medellín - Facultad de Ciencias
2012
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| Online Access: | https://revistas.unal.edu.co/index.php/rfc/article/view/49046 |
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