Effect of gamma rays on vegetative buds of Physalis peruviana L
In Colombia, studies carried out to establish modern methods for propagation and selection of plant of ribes gooseberry (Physalis peruviana) are scarce so, it is necessary to explore alternative techniques such as the application of ionizing radiation, which enables greater genetic variability and to obtain seedlings with phenotypic characteristics that could be beneficial to contribute to improve productivity. To induce chromosomal alterations, axillary buds of P. peruviana with 50, 100, :00 and M00 Gy of gamma radiation were irradiated and were cultured in MS medium supplemented with BA 0.1 mg•L-1 plus IBA 0.05 mg•L-1. T4e experiment was con- ducted using a complete random design, Vve tests each one witht thirty experimental units were carried out. After nine subcultures NY multiplication cycles, M0 days each), regenerated shoots of irradiated buds presented smaller viability than the regenerated non irradiated buds. The regenerated plantlets buds irradiated with 100 Gy performed higher rooting percentage, shoot length and number of leaves , the lethal dose of M00 Gy inhibited the development of shoots and roots, althougt the explants remained viable. In cells taken from radical tips of regenerated plantlets of irradiated buds were observed bridges in anap4ase and telop4ase, laggards and isolated chromosomes, being higher the frequency of laggard chromosomes. The higher percentage of cells with chromosomal alterations was quantified in radical tips of regenerated plantlets of buds irradiated with :00 Gy. In greenhouse conditions, 86-YM.8Z of regenerated plantlets of irradiated or not buds were viable.Key words: cromosomal alterations, gamma radiation, in vitro culture, ionizing radiation, Physalis, plantlets.
| Main Authors: | , , , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Universidad Nacional de Colombia - Sede Palmira
2012
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| Online Access: | https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/38128 |
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