Regeneration of coffee-shading Walnut (Cordia alliodora (Ruiz and Pav.) Oken) from indirect organogenesis
This study, using indirect organogenesis from cotyledonal leaf evaluated Cordia alliodora (Ruiz y Pav) Oken regeneration, a tree native to Colombia with industrial, medical and aesthetic properties. The seeds were first treated with 3% sodium hypochlorite solution during 15 minutes supplemented withPPM® added to the culture medium to remove any bacteria and fungi. Three hormones were tested in the induction phase: BAP, Picloram and 2,4-D in concentrations from 0,5 to 12,5 mg/L in light and dark conditions; production of callus was obtained in all of them. After thirty days most of the callus were oxidized; some callus on BAP 10mg/L showed organogenic shoots. These results suggest a way for in vitro regeneration through indirect organogenesis from coffee-shading walnut cotyledonal leaves and suggest that more research proceed in evaluating this regeneration technique and other in vitro regeneration techniques.
| Main Authors: | , |
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| Format: | Digital revista |
| Language: | eng spa |
| Published: |
Universidad Nacional de Colombia - Sede Palmira
2013
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| Online Access: | https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/31342 |
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