Assessing the in vitro effect of a bacterial biopreparation on coffee trees (Coffea canephora P.)
This study was carried out at the Cuban National Institute of Agricultural Sciences to evaluate the effect of several bacterial compound BC-1 concentrations on the growth and in vitro development of three selected coffee tree clones (Robusta variety), as well as to evaluate the possible substitution of AIA auxin in coffee tree propagation culture medium. Testing was done during vitroplant acclimation. Different treatments were studied; conventionally established propagation medium was used as a check, whilst the other three treatments consisted of substituting AIA from the medium for the CB-1 compound in several concentrations (0.5, 1.0 and 2.0 mg.L-1). The biopreparation's (liquid and solid) effect was studied during acclimation by exposing plants' root systems to a 20 mg/L compound concentration for 30 minutes. Variables evaluated were: number of living explants, vitroplant height (cm), number of shoots, number of leaf pairs, percentage of rooted plants and total protein and phenol content (tissue mg/g). Variations were detected in the response of those clones studied, as well as in that of those indicators evaluated, the best results being obtained with the M-229 clone at 0.5 mg/L BC-1 concentration. The greatest effect was obtained with the liquid biopreparation during vitroplant acclimation phase. This new biopreparation's use in vegetable growth stimulation was shown to be feasible.
| Main Authors: | , , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Universidad Nacional de Colombia - Sede Bogotá - Instituto de Biotecnología
2001
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| Online Access: | https://revistas.unal.edu.co/index.php/biotecnologia/article/view/30075 |
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