Correlation between the presence of both gross and histopathological lesions of enzootic pneumonia, and detection of Mycoplasma hyopneumoniae in bronchoalveolar lavage fluids by nested-PCR of slaughter pigs.
Mycoplasma hyopneumoniae is primary etiologic agent of Enzootic Pneumonia of Swine (EPS), word wide spread, and is the most prevalent and important infectious agent involved in the porcine respiratory complex (PRD C). The purpose of this study is recording the lesions of 55 pigs at slaughter plant from 5 farms of swine herds, aleatory sampled for histopathology and detection of DNA of Mycoplasma hyopneumoniae in bronchoalveolar lavages fluids by nested-PCR technique, and statistic study to correlation. The criterion for lungs sampling is based on suggestive lesions of EPS, that consist of craneo-ventral bronchopneumonic areas, and grossly normal lungs to controls that were processed in ICACEISA Laboratories, Bogotá. The results of nested-PCR were scored positive or negative, and gross lesions were graded. The histopathological lesions were graded 0-4, according to the M. hyopneumoniae-like lesions as BALT hyperplasia, epithelial cells hyperplasia, infiltration of inflammatory cells into the airways, emphysema, haemorrhage, atelectasia, alveolar and interlobulillar septal thickening, with category 0 for lungs without lesions and category 4 for lungs with severe lesions. Nested-PCR was positive in 54 of 55 samples. The BALT histopathological lesions showed high correlation with epithelial cell hyperplasia, infiltration of inflammatory cells into airways, as slaughter finding. The results demonstrate that nested-PCR is a valuable complement diagnosis to the presence of Mycoplasma hyopneumoniae in respiratory affections of Enzootic Pneumonia or PRD C in slaughter pigs.
| Main Authors: | , , , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Universidad Nacional de Colombia - Sede Bogotá - Facultad de Medicina Veterinaria y de Zootecnia
2008
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| Online Access: | https://revistas.unal.edu.co/index.php/remevez/article/view/10536 |
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