Cholesterol modulates acetylcholine receptor diffusion by tuning confinement sojourns and nanocluster stability
Abstract: Translational motion of neurotransmitter receptors is key for determining receptor number at the synapse and hence, synaptic efficacy. We combine live-cell STORM superresolution microscopy of nicotinic acetylcholine receptor (nAChR) with single-particle tracking, mean-squared displacement (MSD), turning angle, ergodicity, and clustering analyses to characterize the lateral motion of individual molecules and their collective behaviour. nAChR diffusion is highly heterogeneous: subdiffusive, Brownian and, less frequently, superdiffusive. At the single-track level, free walks are transiently interrupted by ms-long confinement sojourns occurring in nanodomains of ~36 nm radius. Cholesterol modulates the time and the area spent in confinement. Turning angle analysis reveals anticorrelated steps with time-lag dependence, in good agreement with the permeable fence model. At the ensemble level, nanocluster assembly occurs in second-long bursts separated by periods of cluster disassembly. Thus, millisecond-long confinement sojourns and second-long reversible nanoclustering with similar cholesterol sensitivities affect all trajectories; the proportion of the two regimes determines the resulting macroscopic motional mode and breadth of heterogeneity in the ensemble population.
| Main Authors: | , , |
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| Format: | Artículo biblioteca |
| Language: | eng eng |
| Published: |
Nature Research
2018
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| Subjects: | COLESTEROL, MOLECULAS, LIPIDOS, NEUROTRANSMISORES, CEREBRO, TRANSMISIÓN SINAPTICA, |
| Online Access: | https://repositorio.uca.edu.ar/handle/123456789/1464 |
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