Callus induction and pro-embryogenic mass formation in Myrciaria dubia, an important medicinal and nutritional plant

Abstract: Caçari is a fruit tree that is native to the Amazon region of Brazil. The fruit is considered to have the highest vitamin C content of any edible fruit (13.757 mg 100 g-1 dry weight). The objective of this study was to evaluate the effects of 2,4-D and 6-BAP alone or in combination on in vitro callus induction and pro-embryogenic mass formation in caçari stem segments in different culture media as a precursor for caçari micropropagation. The experiment consisted of two culture media (MS and WPM) and combinations of four concentrations of 2,4-D (0, 1, 2 and 4 mg L-1) and BAP (0, 0.25, 0.5 and 1 mg L-1). The results showed that the highest percentage of callus formation (99%) was obtained in WPM supplemented with 4 mg L-1, 2,4-D and 1 mg L-1 BAP, and this combination resulted in 93% pro-embryogenic mass formation.

Bibliographic Details

Main Authors:	Araújo,Maria da Conceição da Rocha, Chagas,Edvan Alves, Vendrame,Wagner, Ribeiro,Maria Isabel Garcia, Moura,Elias Ariel de, Taveira,Daniel Lucas Lima, Chagas,Pollyana Cardoso, Grigio,Maria Luiza
Format:	Digital revista
Language:	English
Published:	Crop Breeding and Applied Biotechnology 2021
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000300200
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