Cytological characterization of Jatropha curcas callus in different periods of cultivation

The aimed was characterization and determining the time for Jatropha curcas callus subculture. The pattern of J. curcas callus development is sigmoidal with 5 distinct phases, and transplantation should be performed at the end of the deceleration phase (approximately day 28). During callus development, it was observed that at the onset of growth in the exponential phase the cells were internally disorganized; in the linear phase, respiratory metabolism was resumed through reorganization of the first mitochondria; and by the mid-deceleration phase, the cells were entirely active, and several organelles were detected. This organizational status was maintained throughout the stationary phase during which somatic pre-embryos were identified. At the end of the stationary phase, intracellular disruption began, and the cells entered senescence, which characterized the decline phase for the J. curcas calli growth curve.

Bibliographic Details

Main Authors:	Santos,Dalilhia Nazaré dos, Nunes,Claudinéia Ferreira, Soares,Joyce Dória Rodrigues, Valente,Thaís Cainã Teixeira, Alves,Eduardo, Labory,Cláudia Regina Gontijo, Pasqual,Moacir
Format:	Digital revista
Language:	English
Published:	Crop Breeding and Applied Biotechnology 2013
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332013000400002
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