First report of detection of Toxoplasma gondii DNA in oysters (Crassostrea sp.) in the state of Maranhão

Abstract The aim of this study was to report on detection of Toxoplasma gondii DNA in oysters (Crassostrea sp.) in the state of Maranhão. To conduct this study, 200 farmed oysters were acquired in the municipality of Raposa and 100 in Paço do Lumiar; and a further 100 oysters were taken from the natural stock in the municipality of Primeira Cruz. This total of 400 specimens sampled was divided into 80 pools composed of five animals each. The gills and visceral mass of each oyster were removed for DNA extraction (per pool of oysters), using a commercial kit. The nested PCR technique (with the primer SAG-1) was then used to investigate any presence of protozoa. This molecular technique demonstrated the presence of DNA of T. gondii in 2.5% of the pools of oysters (n = 2/80): these oysters were exclusively from farms. The results from this study allow the conclusion that oysters of the genus Crassostrea that are farmed in the state of Maranhão are capable of filtering oocysts of T. gondii and maintaining them in their tissues. They are therefore potential sources of contamination for humans and other animals.

Bibliographic Details

Main Authors:	Silva,Camila Moraes, Silva,Anna Letícia Pinto, Watanabe,Karinne Francisca Cardoso, Chaves Bezerra,Nancyleni Pinto, Bezerra,Danilo Cutrim, Gomes,Hugo Moreira, Freire,Thais Brito, Santos,Larissa Sarmento dos, Carvalho Neta,Alcina Vieira de, Silva,Ellainy Maria Conceição, Coimbra,Viviane Correia Silva
Format:	Digital revista
Language:	English
Published:	Colégio Brasileiro de Parasitologia Veterinária 2020
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612020000300402
Tags:	Add Tag No Tags, Be the first to tag this record!