Hypothalamic endoplasmic reticulum stress of overtrained mice after recovery

Abstract AIMS knowing the relationship between endoplasmic reticulum (ER) stress and inflammation and based on the fact that downhill running-based overtraining (OT) model increases hypothalamus levels of some pro-inflammatory cytokines, we verified the effects of three OT protocols on the levels of BiP, pIRE-1 (Ser734), pPERK (Thr981), pelF2alpha (Ser52), ATF-6 and GRP-94 proteins in the mouse hypothalamus after two weeks of recovery. METHODS the mice were randomized into control (CT), overtrained by downhill running (OTR/down), overtrained by uphill running (OTR/up) and overtrained by running without inclination (OTR) groups. After 2-week total recovery period (i.e., week 10), hypothalamus was removed and used for immunoblotting. RESULTS the OTR/down group exhibited high levels of BiP and ATF6. The other OT protocols showed higher levels of pPERK (Th981) and pelf-2alpha (Ser52) when compared with the CT group. CONCLUSION the current results suggest that after a 2-week total recovery period, the overtrained groups increased partially their ER stress protein levels, but without hypothalamic inflammation, which characterizes a physiological condition related to an adaptation mechanism.

Bibliographic Details

Main Authors:	Pinto,Ana P., Oliveira,Luciana da C., Rocha,Alisson L. da, Pereira,Bruno C., Morais,Gustavo P., Vicente,Larissa Gaioto de, Moura,Leandro P., Pauli,José R., Silva,Adelino S. R. da
Format:	Digital revista
Language:	English
Published:	Universidade Estadual Paulista 2017
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1980-65742017000500302
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