Peroxidase activity in roots of arracacha affected by pH and temperature

Peroxidase activity in roots of arracacha affected by pH and temperature

In this paper, roots of arracacha (Arracacia xanthorrhyza Bancroft) were stored at 5ºC to induce chilling injury symptoms and stress-related peroxidase activity. Later, peroxidase kinetic activity was determined in different pH and temperature conditions. For this, soluble crude extract was sequentially saturated with ammonium sulfate, obtaining a semi-purified enzyme solution used for the analysis. Activity of peroxidase induced by the chilling at 5º C was determined from pH 2.5 to 9.0 and at temperature ranging from 10 to 80º C. The peroxidase had higher activity when the reaction occurred between pH 5.5 and 6.0 and at temperature of 30º C. Complete inactivation of the activity was observed in pH 2.5 after 60 minutes of pre-incubation or at 60º C for 10 minutes or alternatively at 70º C after 5 minutes of pre-incubation. The enzyme is more susceptible to inactivation in acid than alkaline pHs or alternatively using heat treatment.

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Main Authors: Menolli,Luciana Nunes, Finger,Fernando Luiz, Barbosa,Janaína Miranda, Correia,Teresa Drummond, Vieira,Luciana Marques
Format: Digital revista
Language:English
Published: Editora da Universidade Estadual de Maringá - EDUEM 2011
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-86212011000300019
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spelling oai:scielo:S1807-862120110003000192011-07-21Peroxidase activity in roots of arracacha affected by pH and temperatureMenolli,Luciana NunesFinger,Fernando LuizBarbosa,Janaína MirandaCorreia,Teresa DrummondVieira,Luciana Marques Arracacia xanthorrhyza Bancroft oxidative enzyme inactivation In this paper, roots of arracacha (Arracacia xanthorrhyza Bancroft) were stored at 5ºC to induce chilling injury symptoms and stress-related peroxidase activity. Later, peroxidase kinetic activity was determined in different pH and temperature conditions. For this, soluble crude extract was sequentially saturated with ammonium sulfate, obtaining a semi-purified enzyme solution used for the analysis. Activity of peroxidase induced by the chilling at 5º C was determined from pH 2.5 to 9.0 and at temperature ranging from 10 to 80º C. The peroxidase had higher activity when the reaction occurred between pH 5.5 and 6.0 and at temperature of 30º C. Complete inactivation of the activity was observed in pH 2.5 after 60 minutes of pre-incubation or at 60º C for 10 minutes or alternatively at 70º C after 5 minutes of pre-incubation. The enzyme is more susceptible to inactivation in acid than alkaline pHs or alternatively using heat treatment.info:eu-repo/semantics/openAccessEditora da Universidade Estadual de Maringá - EDUEMActa Scientiarum. Agronomy v.33 n.3 20112011-09-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-86212011000300019en10.4025/actasciagron.v33i3.6264
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Menolli,Luciana Nunes
Finger,Fernando Luiz
Barbosa,Janaína Miranda
Correia,Teresa Drummond
Vieira,Luciana Marques
spellingShingle Menolli,Luciana Nunes
Finger,Fernando Luiz
Barbosa,Janaína Miranda
Correia,Teresa Drummond
Vieira,Luciana Marques
Peroxidase activity in roots of arracacha affected by pH and temperature
author_facet Menolli,Luciana Nunes
Finger,Fernando Luiz
Barbosa,Janaína Miranda
Correia,Teresa Drummond
Vieira,Luciana Marques
author_sort Menolli,Luciana Nunes
title Peroxidase activity in roots of arracacha affected by pH and temperature
title_short Peroxidase activity in roots of arracacha affected by pH and temperature
title_full Peroxidase activity in roots of arracacha affected by pH and temperature
title_fullStr Peroxidase activity in roots of arracacha affected by pH and temperature
title_full_unstemmed Peroxidase activity in roots of arracacha affected by pH and temperature
title_sort peroxidase activity in roots of arracacha affected by ph and temperature
description In this paper, roots of arracacha (Arracacia xanthorrhyza Bancroft) were stored at 5ºC to induce chilling injury symptoms and stress-related peroxidase activity. Later, peroxidase kinetic activity was determined in different pH and temperature conditions. For this, soluble crude extract was sequentially saturated with ammonium sulfate, obtaining a semi-purified enzyme solution used for the analysis. Activity of peroxidase induced by the chilling at 5º C was determined from pH 2.5 to 9.0 and at temperature ranging from 10 to 80º C. The peroxidase had higher activity when the reaction occurred between pH 5.5 and 6.0 and at temperature of 30º C. Complete inactivation of the activity was observed in pH 2.5 after 60 minutes of pre-incubation or at 60º C for 10 minutes or alternatively at 70º C after 5 minutes of pre-incubation. The enzyme is more susceptible to inactivation in acid than alkaline pHs or alternatively using heat treatment.
publisher Editora da Universidade Estadual de Maringá - EDUEM
publishDate 2011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-86212011000300019
work_keys_str_mv AT menolliluciananunes peroxidaseactivityinrootsofarracachaaffectedbyphandtemperature
AT fingerfernandoluiz peroxidaseactivityinrootsofarracachaaffectedbyphandtemperature
AT barbosajanainamiranda peroxidaseactivityinrootsofarracachaaffectedbyphandtemperature
AT correiateresadrummond peroxidaseactivityinrootsofarracachaaffectedbyphandtemperature
AT vieiralucianamarques peroxidaseactivityinrootsofarracachaaffectedbyphandtemperature
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