Peroxidase activity in roots of arracacha affected by pH and temperature
In this paper, roots of arracacha (Arracacia xanthorrhyza Bancroft) were stored at 5ºC to induce chilling injury symptoms and stress-related peroxidase activity. Later, peroxidase kinetic activity was determined in different pH and temperature conditions. For this, soluble crude extract was sequentially saturated with ammonium sulfate, obtaining a semi-purified enzyme solution used for the analysis. Activity of peroxidase induced by the chilling at 5º C was determined from pH 2.5 to 9.0 and at temperature ranging from 10 to 80º C. The peroxidase had higher activity when the reaction occurred between pH 5.5 and 6.0 and at temperature of 30º C. Complete inactivation of the activity was observed in pH 2.5 after 60 minutes of pre-incubation or at 60º C for 10 minutes or alternatively at 70º C after 5 minutes of pre-incubation. The enzyme is more susceptible to inactivation in acid than alkaline pHs or alternatively using heat treatment.
| Main Authors: | , , , , |
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| Format: | Digital revista |
| Language: | English |
| Published: |
Editora da Universidade Estadual de Maringá - EDUEM
2011
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| Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-86212011000300019 |
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