Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma

OBJECTIVE: To establish the method of isolation and culture of human glioblastoma neurospheres, and the purification of their stem cells, followed by the process of obtaining tumor subspheres, immunophenotypically characterizing this clonogenic set. METHODS: Through the processing of glioblastoma samples (n=3), the following strategy of action was adopted: (i) establish primary culture of glioblastoma; (ii) isolation and culture of tumor neurospheres; (iii) purify cells that initiate tumors (CD133+) by magnetic separation system (MACS); (iv) obtain tumor subspheres; (v) study the expression of the markers nestin, CD133, and GFAP. RESULTS: The study successfully described the process of isolation and culture of glioblastoma subspheres, which consist of a number of clonogenic cells immunophenotypically characterized as neural, which are able to initiate tumor formation. CONCLUSION: These findings may contribute to a better understanding of the process of gliomagenesis.

Bibliographic Details

Main Authors:	Pavon,Lorena Favaro, Marti,Luciana Cavalheiro, Sibov,Tatiana Tais, Miyaki,Liza Aya Mabuchi, Malheiros,Suzana Maria Fleury, Mamani,Javier Bustamante, Brandt,Reynaldo Andre, Ribas,Guilherme Carvalhal, Pagura,Jorge Roberto, Joaquim,Marcos Augusto Stavale, Feres Junior,Hallin, Gamarra,Lionel Fernel
Format:	Digital revista
Language:	English
Published:	Instituto Israelita de Ensino e Pesquisa Albert Einstein 2012
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082012000200013
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