Genotypic analysis of secreted aspartyl proteinases in vaginal Candida albicans isolates

ABSTRACT Introduction: Candida albicans is the most common etiologic agent of fungal vaginitis. These yeasts produce secreted aspartyl proteinases encoded by a family of 10 genes (SAP1-10). Objective: The purpose of this study was to analyze the presence of genes SAP1-7 in vulvovaginal C. albicans. Materials and method: The study included 26 C. albicans vaginal isolates. Detection of aspartyl proteases genes (SAP1-7) was performed by polymerase chain reaction (PCR). Results: The most frequent gene in C. albicans isolated from colonization was SAP6 (93.33%), and from infection, SAP7 (100%). We observed a statistical difference (p = 0.049) in SAP1 gene frequency between isolates from vulvovaginal colonization and infection. Conclusion: High frequency of SAP genes was observed in vulvovaginal C. albicans. The results suggest SAP1 participation in vulvovaginal candidiasis infection.

Bibliographic Details

Main Authors:	Lima,Janaína S., Braga,Kaoane Regina G. S., Vieira,Camila A., Souza,Wérika Weryanne R., Chávez-Pavoni,Juliana Helena, Araújo,Claudinéia de, Goulart,Letícia S.
Format:	Digital revista
Language:	English
Published:	Sociedade Brasileira de Patologia Clínica 2018
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442018000100028
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