Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic

Gastric cancer (GC) is a multifactorial disease with a high mortality rate in Brazil and worldwide. This work aimed to evaluate single nucleotide polymorphisms (SNP) rs1695, in the Glutathione S-Transferase Pi (GSTP1) gene in GC samples by comparative analysis Specific PCR - ASP and Dideoxy Single Allele-Specific PCR - DSASP methods. The DSASP is the proposed new method for allelic discrimination. This work analyzed 60 GC samples, 26 diffuse and 34 intestinal types. The SNP rs1695 of the GSTP1 gene was significantly associated with GC analyzed by DSASP method (χ2 = 9.7, P < 0.05). A comparative analysis of the data obtained from both methods did not differ significantly (χ2 = 0.08, P > 0.05). These results suggest that the SNP rs1695 of the GSTP1 gene was a risk factor associated with gastric carcinogens is and the DSASP method was a new successfully low-cost strategy to study allelic discrimination.

Bibliographic Details

Main Authors:	Lima,Eleonidas Moura, Lopes,Otávio Sérgio, Soares,Leonardo Ferreira, Arruda,Talitta Dantas, Gigek,Carolina Oliveira, Melo,Cynthia Germoglio Farias, Smith,Marilia Arruda Cardoso, Oliveira,João Ricardo Gonçalves, Medeiros,Arnaldo, Delatorre,Plínio, Burbano,Rommel Rodriguez
Format:	Digital revista
Language:	English
Published:	Instituto de Tecnologia do Paraná - Tecpar 2015
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300414
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