Molecular cloning of αRYR hotspot region 1 from broiler chicken

Samples of Pectoralis major m. were collected, and an RT-PCR analysis of the a-Ryanodine receptor (a RYR) from chicken mRNA hotspot region spanning aminoacid residues 386 to 540, numbered according to the turkey sequence, revealed two classes of transcripts. The sequences of the first class were similar to turkey and human with 97% and 74% of identity, respectively, and included all transcripts with substitutions in the nucleotide sequence. The second class was characterized by the deletion of nucleotides, leading to a premature stop codon and coding for a truncated and nonfunctional protein. These results are to date the first report related to the sequencing of the chicken αRYR hotspot region 1, which will possibility serve as a guide for further studies regarding a solution in the poultry production chain related to the problem of pale, soft and exudative (PSE) meat.

Bibliographic Details

Main Authors:	Ziober,Iris Lamberti, Paião,Fernanda Gonzales, Marin,Silvana Regina Rockenbach, Marchi,Denis Fabricio, Binneck,Eliseu, Nepomuceno,Alexandre Lima, Coutinho,Luiz Lehmann, Shimokomaki,Massami
Format:	Digital revista
Language:	English
Published:	Instituto de Tecnologia do Paraná - Tecpar 2009
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700029
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