Production of specific polyclonal antibodies anti-human factor IX

Production of specific polyclonal antibodies anti-human factor IX

In this work, polyclonal antibodies anti-human Factor IX were produced in New Zealand rabbits by immunization with commercial pure human FIX (hFIX) (Octanyne®, Octapharma, USA). The serum containing immunoglobulins anti-hFIX was useful to detect hFIX antigen in human plasma fractions submitted to anionic exchange chromatographic process and with a large yield. Immunoassays (ELISA) using bovine serum albumin, trypsin and peptides generated by cleavage assays with trypsin as digestion enzyme was performed and revealed adequate specificity of the polyclonal antibodies produced.

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Bibliographic Details
Main Authors: Chaves,Daniel Gonçalves, Rodrigues,Cibele Velloso, Ferreira,Wanderley Almeida, Siqueira,Pollyana Fantini Miranda, Duarte,Clara Guerra, Carneiro-Proietti,Anna Bárbara de Freitas, Santoro,Marcelo Matos
Format: Digital revista
Language:English
Published: Instituto de Tecnologia do Paraná - Tecpar 2006
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500010
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