Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system
Studies were carried out on the partition of amylase from Bacillus subtilis in a minimal medium at 37 ºC and 110 rpm. Enzyme recovery was carried out in aqueous two-phase system PEG-Phosphate salt were carried out. The best purification factor (5.4) was obtained in system PEG 1000 (16.7% w/w) with potassium phosphate (14.8% w/w), at pH 6.0, resulting in a recovery of 45.2% activity enzymatic in the salt-rich phase.
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| Principais autores: | , , , , , |
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| Formato: | Digital revista |
| Idioma: | English |
| Publicado em: |
Instituto de Tecnologia do Paraná - Tecpar
2006
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| Acesso em linha: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500003 |
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