Mutagenic activity of airborne particulate matter from the urban area of Porto Alegre, Brazil

The mutagenic activity of airborne particulate matter collected from three different sites within the urban area of Porto Alegre, Brazil, was investigated using a Salmonella/microsome assay. Samples were extracted by sonication, sequentially, with cyclohexane (CX), and dichloromethane (DCM), for a rough fractionation by polarity. The different fractions were tested for mutagenicity using Salmonella typhimurium strains TA98, with and without metabolic activation (S9 mix fraction), and TA98NR and TA98/1,8-DNP6, without metabolic activation. Mutagenic response was observed for frameshift strain TA98 in assays with and without metabolization for two sites (sites 2 and 3), which had considerable risk of environmental contamination by nonpolar (CX) and/or moderately polar (DCM) compounds. However, the values of revertants/m3 (rev/m3) were highest on the site subject to automobile exhaust (site 3) in assays without (9.56 rev/m3) and with metabolization (5.08 rev/m3). Maximum mutagenic activity was detected in the moderately polar fraction, decreasing after metabolization. Nevertheless, the nonpolar fractions (CX) gave higher mutagenic activity in the presence of metabolization than in the absence of the S9 mix fraction. The responses observed for TA98NR and TA98/1,8-DNP6 strains suggest the activity of nitrocompounds.

Bibliographic Details

Main Authors:	Vargas,Vera Maria Ferrão, Horn,Rubem Cesar, Guidobono,Régis Rolim, Mittelstaedt,Ana Beatriz, Azevedo,Irascema Girardi de
Format:	Digital revista
Language:	English
Published:	Sociedade Brasileira de Genética 1998
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200013
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