Detection of group A beta-hemolytic Streptococcus employing three different detection methods: culture, rapid antigen detecting test, and molecular assay
In order to study the prevalence of Group A beta-hemolytic Streptococcus (GABHS) pharyngotonsillitis in our pediatric population and to compare different sampling methods of GABHS detection, oropharyngeal swabs from 50 children with acute pharyngotonsillitis, between 1 and 12 years old, were used simultaneously for culture, molecular assay and rapid GABHS antigen detection tests. All children were clinically examined at the Division of Pediatric Otorhinolaryngology of the Federal University of São Paulo. Diagnostic criteria were based on signs and symptoms, including sore throat, fever and oropharyngeal purulent secretion. Children that had been treated with antibiotics were excluded. Overall, combining the three methods, the prevalence of GABHS was 34%. GABHS was diagnosed in 30% of the bacterial cultures, in 25% of the samples tested with the molecular nucleic acid hybridization method and in 26% of the cases tested with the rapid antigen detection test. There was no significant difference between these three methods.
Main Authors: | Santos,Odimara, Weckx,Luc Louis Maurice, Pignatari,Antonio Carlos Campos, Pignatari,Shirley Shizue Nagata |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Brazilian Society of Infectious Diseases
2003
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000500003 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Detection of human papilloma virus in the tonsils of children undergoing tonsillectomy
by: Ribeiro,Kátia Moreira Xavier, et al.
Published: (2006) -
Rapid Detection of Sepsis using CESDA: the Caenorhabditis elegans Sepsis Detection Assay
by: Tee,Ling Fei, et al.
Published: (2019) -
Phage amplification assay as rapid method for Salmonella detection
by: Siqueira,Regina Silva de, et al.
Published: (2003) -
Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen
by: Illnait,Ma.Teresa, et al.
Published: (2001) -
Estudo microbiológico do core e superfície das amígdalas palatinas em crianças portadoras de faringoamigdalites de repetição e hipertrofia adenoamigdaliana
by: Costa,Felipe Neiva, et al.
Published: (2003)