Survival and mutant production induced by mutagenic agents in Metarhizium anisopliae
A wild strain of Metarhizium anisopliae, an entomopathogenic fungus, was submitted to three mutagenic agents: gamma radiation, ultraviolet light and nitrous acid. Survival curves were obtained and mutants were selected using different mutagenic doses which gave 1 to 5% survival. Morphological and auxotrophic mutants were isolated. Morphological mutants were grouped in a class with yellow conidia and other with pale vinaceous conidia as opposed to the green wild type conidia. Auxotrophic mutants had requirements for vitamin and aminoacid biosynthesis. More than 58% of the total auxotrophk mutants required proline/aipnine. Gamma radiation showed to be the most efficient mutagenic agent giving 0.2% of auxotrophk mutants followed by ultraviolet light (0.12%) and nitrous acid (0.06%).The conidial colour and auxotrophk mutants isolated until now from M. anisopliae were reviewed.
| Main Authors: | , , |
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| Format: | Digital revista |
| Language: | English |
| Published: |
Escola Superior de Agricultura "Luiz de Queiroz"
1995
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| Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161995000300023 |
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