Cholinesterases Inhibition by Novel cis- and trans-3-Arylaminocyclohexyl N,N-Dimethylcarbamates: Biological Evaluation and Molecular Modeling

The present study describes the synthesis, assessment of the anticholinesterase activity and the inhibition type of novel cis- and trans-3-arylaminocyclohexyl N,N-dimethylcarbamates. In vitro inhibition assay by Ellman's method with human blood samples showed that carbamates were selective for butyrylcholinesterase (BuChE) with compound concentration that inhibits 50% of enzyme activity (IC50) between 0.11 and 0.18 mmol L-1. cis- and trans-3-(4-Methoxyphenylamino)cyclohexyl N,N-dimethylcarbamate hydrochloride were the most active for BuChE, showing that the presence of methoxyl group enhanced the anticholinesterase activity. The enzyme kinetics studies indicate a noncompetitive inhibition against acetylcholinesterase (AChE) and mixed type inhibition for BuChE. Molecular modeling studies confirm the ability of carbamates to bind both the active and peripheral sites of the BuChE.

Detalhes bibliográficos

Principais autores:	Yamazaki,Diego A. S., Cândido,Augusto A., Bagatin,Mariane C., Machinski Jr.,Miguel, Mossini,,Simone A. G., Pontes,Rodrigo M., Rosa,Fernanda A., Basso,Ernani A., Gauze,Gisele F.
Formato:	Digital revista
Idioma:	English
Publicado em:	Sociedade Brasileira de Química 2016
Acesso em linha:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532016000901616
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