Method validation for simultaneous determination of cholesterol and cholesterol oxides in milk by RP-HPLC-DAD
The aim of the present work was to develop and validate a reversed-phase highperformance liquid chromatography with diode array detection (RP-HPLC-DAD) method for determination of cholesterol and cholesterol oxides extracted from milk samples. The method consisted of direct samples saponification and analytes extraction, followed by their determination using RP-HPLC-DAD. Suitable analytical selectivity, linearity and range of application, intra-assay and intermediary precision, recovery, detection and quantification limits (LOD and LOQ) were achieved and, the results showed that the developed RP-HPLC-DAD method can be applied to routine determination of cholesterol and cholesterol oxides in milk samples.
Main Authors: | , , , , , |
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Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira de Química
2014
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Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000100020 |
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