Acute pulmonary injury induced by experimental muscle trauma

PURPOSE: To develop an easily reproducible model of acute lung injury due to experimental muscle trauma in healthy rats. METHODS: Eighteen adult Wistar rats were randomized in 3 groups (n=6): G-1- control, G-2 - saline+trauma and G-3 - dexamethasone+trauma. Groups G-1 and G-2 were treated with saline 2,0ml i.p; G-3 rats were treated with dexamethasone (DE) (2 mg/kg body weight i.p.). Saline and DE were applied 2h before trauma and 12h later. Trauma was induced in G-2 and G-3 anesthetized (tribromoethanol 97% 100 ml/kg i.p.) rats by sharp section of anterior thigh muscles just above the knee, preserving major vessels and nerves. Tissue samples (lung) were collected for myeloperoxidase (MPO) assay and histopathological evaluation. RESULTS: Twenty-four hours after muscle injury there was a significant increase in lung neutrophil infiltration, myeloperoxidase activity and edema, all reversed by dexamethasone in G-3. CONCLUSION: Trauma by severance of thigh muscles in healthy rats is a simple and efficient model to induce distant lung lesions.

Bibliographic Details

Main Authors:	Sombra,Márcia Andréa da Silva Carvalho, Vasconcelos,Marcelo Pinho Pessoa de, Guimarães,Sergio Botelho, Escalante,Rodrigo Dornfeld, Garcia,José Huygens Parente, Vasconcelos,Paulo Roberto Leitão de
Format:	Digital revista
Language:	English
Published:	Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2011
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502011000700009
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