A new experimental model for acute hepatic failure in rats

PURPOSE: To develop a reliable surgical model of acute hepatic failure and hyperammonemia in rats that avoids porto-systemic shunt and bile duct ligation, applicable to hepatic encephalopathy research. METHODS: The pedicles of right lateral and caudate lobes were exposed and clamped. One hour later, the animal was reopened, clamps were released and anterior subtotal hepatectomy (resection of median and left lateral lobes) was performed, comprising 75% of liver removal. Four hours after hepatectomy, blood samples and liver tissues were collected from ALF and control groups. RESULTS: Differences between ALF and control groups were significant for ALT, AST, total and direct bilirubin, sodium, potassium, alkaline phosphatasis, gamma-glutamyltransferase and most important, ammonia. Histologically, significant differences were noticed between groups. CONCLUSION: The model is useful for the study of specific aspects of ALF and the development of new therapeutic approaches.

Detalles Bibliográficos

Autores principales:	Teixeira,Antonio Roberto Franchi, Machado,Marcel Cerqueira Cesar, Kubrusly,Márcia Saldanha, Molan,Nilza Trindade, Bellodi-Privato,Marta, Leite,Kátia Regina, D'Albuquerque,Luiz Augusto Carneiro
Formato:	Digital revista
Idioma:	English
Publicado:	Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2010
Acceso en línea:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000300009
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