Experimental model for fibroblast culture

The use of cell culture methods in Plastic Surgery opened a new horizon in the research of cellular mechanisms of proliferation and biosynthesis functions. Several types of cells have been investigated in the cutaneous compartment. Keratinocytes and fibroblasts have been studied aiming the possibility of developing biomaterial for skin substitution. The present study describes the standardization for the development of fibroblast primary culture, its utilization in experiments and its storage.

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Bibliographic Details
Main Authors: Keira,Sidney Mamoru, Ferreira,Lydia Masako, Gragnani,Alfredo, Duarte,Ivone da Silva, Santos,Isabel Anunciação Neves dos
Format: Digital revista
Language:English
Published: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2004
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502004000700004
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