A dehydrorotenoid produced by callus tissue culture and wild plant roots of Boerhaavia coccinea

Calli cultures were established from leaves and stem of B. coccinea plantlet produced in vitro and analysed for isoflavonoid content. The quantification of 6,9,11-trihydroxy-6a,12a-dehydrorotenoid isolated from the roots of Boerhaavia coccinea P. Miller collected from its natural environment, and the same metabolite produced in callus tissue culture of the same plant are described in this paper. The rotinary quantitative HPLC analysis indicated that callus culture produced the same isoflavonoid compound found in the roots of intact wild growing plant. The amount of the secondary metabolite produced in vitro was 955.35 µg/g of dry cell weight, 2.5 times more than the highest amount concentration produced by the wild growing plant in its natural environment.

Bibliographic Details

Main Authors:	Santos,Alberdan Silva, Araújo,Silvana Fernades, Goulart,Henrique Fonseca, Caetano,Luiz Carlos, Arruda,Mara Silvia Pinheiro, Santos,Lourivaldo da Silva, Santa’Ana,Antônio Euzébio Goulart
Format:	Digital revista
Language:	English
Published:	Sociedade Brasileira de Farmacognosia 2007
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2007000400011
Tags:	Add Tag No Tags, Be the first to tag this record!