Interferon-γ inhibits group B Streptococcus survival within human endothelial cells

Endothelial dysfunction is a major component of the pathophysiology of septicaemic group B Streptococcus (GBS) infections. Although cytokines have been shown to activate human umbilical vein endothelial cells (HUVECs), the capacity of interferon (IFN)-γ to enhance the microbicidal activity of HUVECs against GBS has not been studied. We report that the viability of intracellular bacteria was reduced in HUVECs activated by IFN-γ. Enhanced fusion of lysosomes with bacteria-containing vacuoles was observed by acid phosphatase and the colocalisation of Rab-5, Rab-7 and lysosomal-associated membrane protein-1 with GBS in IFN-γ-activated HUVECs. IFN-γ resulted in an enhancement of the phagosome maturation process in HUVECs, improving the capacity to control the intracellular survival of GBS.

Bibliographic Details

Main Authors:	Lione,Viviane de Oliveira Freitas, Santos,Michelle Hanthequeste Bittencourt dos, Oliveira,Jessica Silva Santos de, Mattos-Guaraldi,Ana Luiza, Nagao,Prescilla Emy
Format:	Digital revista
Language:	English
Published:	Instituto Oswaldo Cruz, Ministério da Saúde 2014
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000700940
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