Prozone effects in microscopic agglutination tests for leptospirosis in the sera of mice infected with the pathogenic Leptospira interrogans serovar Canicola

Mice experimentally infected with a pathogenic strain of Leptospira interrogans serovar Canicola produced false negative results (prozone effect) in a microscopic agglutination test (MAT). This prozone effect occurred in several serum samples collected at different post-infection times, but it was more prominent in samples collected from seven-42 days post-infection and for 1:50 and 1:100 sample dilutions. This phenomenon was correlated with increased antibody titres in the early post-infection phase. While prozone effects are often observed in serological agglutination assays for the diagnosis of animal brucellosis and human syphilis, they are not widely reported in leptospirosis MATs.

Bibliographic Details

Main Authors:	Shimabukuro,Fabio Hiroto, Costa,Veruska Maia da, Silva,Rodrigo Costa da, Langoni,Hélio, Silva,Aristeu Vieira da, Carvalho,Lídia Raquel de, Domingues,Paulo Francisco
Format:	Digital revista
Language:	English
Published:	Instituto Oswaldo Cruz, Ministério da Saúde 2013
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000500668
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