Characterization of insect-specific Culex flavivirus (Flaviviridae) nucleotide sequences in mosquitoes from urban parks in São Paulo, Brazil

ABSTRACT Background: Despite their worldwide occurrence, the distribution and role of insect-specific flaviviruses (ISFs) remain unclear. Methods: We evaluated the presence of ISFs in mosquitoes collected in São Paulo, Brazil, using reverse transcription and semi-nested polymerase chain reaction (PCR). Some of the positive samples were subjected to nanopore sequencing. Results: Twelve mosquito pools (2.8%) tested positive for flavivirus infection. Nanopore sequencing was successfully performed on six samples. Phylogenetic analysis grouped these sequences into genotype 2 of Culex flavivirus (CxFV). Conclusions: The identification of CxFV genotype 2 at new locations in São Paulo highlights the importance of understanding the role of ISFs in mosquito vector competence.

Detalles Bibliográficos

Autores principales:	Coletti,Thaís de Moura, Romano,Camila Malta, Urbinatti,Paulo Roberto, Teixeira,Renildo Souza, Pedrosa,Leila Weiss de Almeida, Nardi,Marcello Schiavo, Natal,Delsio, Costa,Antônio Charlys da, Jesus,Jaqueline Goes de, Claro,Ingra Morales, Sabino,Ester Cerdeira, Witkin,Steven S., Marrelli,Mauro Toledo, Fernandes,Licia Natal
Formato:	Digital revista
Idioma:	English
Publicado:	Sociedade Brasileira de Medicina Tropical - SBMT 2022
Acceso en línea:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822022000100624
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