Enhanced rapid commercial DNA extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: Easy adaptation to current protocols

Abstract INTRODUCTION Herein, the authors describe a simple enhancement to a commercial rapid DNA extraction kit based on simple viral lysis for detecting COVID-19 via RT-qPCR. METHODS After testing several different modifications, the adapted protocol with the best results in preliminary experiments was statistically evaluated in comparison with an automated robotic protocol. RESULTS Processing and testing of 119 nasopharyngeal samples ultimately yielded near-perfect agreement with the automated protocol (κ = 0.981 [95% confidence interval 0.943-1.000]). CONCLUSIONS The low cost and rapidity of the enhanced protocol makes it suitable for adoption in laboratories diagnosing COVID-19, especially those with high demand for examinations.

Bibliographic Details

Main Authors:	Pessoa-e-Silva,Rômulo, Oliveira,Priscilla Stela Santana de, Gonçalves,Sayonara Maria Calado, Guarines,Klarissa Miranda, Carvalho,Lidiane Vasconcelos do Nascimento, Correia,Maria Andreza Bezerra, Rosa,Michelle Melgarejo da, Rêgo,Moacyr Jesus Barreto de Melo, Pitta,Maira Galdino da Rocha, Pereira,Michelly Cristiny
Format:	Digital revista
Language:	English
Published:	Sociedade Brasileira de Medicina Tropical - SBMT 2021
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822021000100657
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