A duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains

Abstract INTRODUCTION: This study aimed to develop a duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains. METHODS: Primers were designed to target the rrs (LG1/LG2) and ligB (LP1/LP2) genes to confirm the presence of the Leptospira genus and the pathogenic species, respectively. RESULTS: The assay showed 100% specificity against 17 Leptospira strains with a limit of detection of 23.1pg/µl of leptospiral DNA and sensitivity of 103 leptospires/ml in both spiked urine and water. CONCLUSIONS: Our duplex endpoint PCR assay is suitable for rapid early detection of Leptospira with high sensitivity and specificity.

Detalles Bibliográficos

Autores principales:	Benacer,Douadi, Zain,Siti Nursheena Mohd, Lewis,John W., Khalid,Mohd Khairul Nizam Mohd, Thong,Kwai Lin
Formato:	Digital revista
Idioma:	English
Publicado:	Sociedade Brasileira de Medicina Tropical - SBMT 2017
Acceso en línea:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822017000200239
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