An optimized one-tube, semi-nested PCR assay for Paracoccidioides brasiliensis detection

Introduction Herein, we report a one-tube, semi-nested-polymerase chain reaction (OTsn-PCR) assay for the detection of Paracoccidioides brasiliensis. Methods We developed the OTsn-PCR assay for the detection of P. brasiliensis in clinical specimens and compared it with other PCR methods. Results The OTsn-PCR assay was positive for all clinical samples, and the detection limit was better or equivalent to the other nested or semi-nested PCR methods for P. brasiliensis detection. Conclusions The OTsn-PCR assay described in this paper has a detection limit similar to other reactions for the molecular detection of P. brasiliensis, but this approach is faster and less prone to contamination than other conventional nested or semi-nested PCR assays.

Bibliographic Details

Main Authors:	Pitz,Amanda de Faveri, Koishi,Andrea Cristine, Tavares,Eliandro Reis, Andrade,Fabio Goulart de, Loth,Eduardo Alexandre, Gandra,Rinaldo Ferreira, Venancio,Emerson Jose
Format:	Digital revista
Language:	English
Published:	Sociedade Brasileira de Medicina Tropical - SBMT 2013
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000600783
Tags:	Add Tag No Tags, Be the first to tag this record!