Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil
INTRODUCTION: West Nile virus (WNV) is a flavivirus with a natural cycle involving mosquitoes and birds. Over the last 11 years, WNV has spread throughout the Americas with the imminent risk of its introduction in Brazil. METHODS: Envelope protein domain III of WNV (rDIII) was bacterially expressed and purified. An enzyme-linked immunosorbent assay with WNV rDIII antigen was standardized against mouse immune fluids (MIAFs) of different flavivirus. RESULTS: WNV rDIII reacted strongly with St. Louis encephalitis virus (SLEV) MIAF but not with other flaviviruses. CONCLUSIONS: This antigen may be a potentially useful tool for serologic diagnosis and may contribute in future epidemiological surveillance of WNV infections in Brazil.
Main Authors: | , , , , , , |
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Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira de Medicina Tropical - SBMT
2013
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Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000100097 |
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