Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans
We report a rapid method for the flow cytometric quantitation of phagocytosis in heparinized complete peripherial blood (HCPB), using commercially available phycoerythrin-conjugated latex particles of 1µm diameter. The method is faster and shows greater reproducibility than Bjerknes' (1984) standard technique using propidium iodide-stained Candida albicans, conventionally applied to the leukocytic layer of peripherial blood but here modified for HCPB. We also report a modification of Bjerknes' Intracellular Killing Test to allow its application to HCPB.
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| Main Authors: | , |
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| Format: | Digital revista |
| Language: | English |
| Published: |
Sociedade Brasileira de Medicina Tropical - SBMT
1997
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| Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821997000600001 |
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