In vitro effects of platelet derived growth factor on human keratocyte response to excimer laser ablation
SUMMARY Purpose: To evaluate the in vitro effects of platelet derived growth factor (PDGF) on the response ofhuman keratocytes to excimer laser ablation. Methods: Keratocytes were cultured to confluence in 6-well tissue culture plates and exposed to an excimer laser beam. After the laser they were mantained in one of the following media: Group I -Dulbecco's Modified Eagle Medium supplemented with heat-inactivated fetal bovine serum (FBS); Group II - DMEM + fetal bovine serum (FBS); Group III - DMEM + heat-inactivated FBS plus PDGF at a concentration of 1 ng/ml and Group IV - DMEM + heat-inactivated FBS plus PDGF at a concentration of 10 ng/ml. To quantitate the rate of wound closure micrographs were taken at O, 24, 48, 72 and 96 hours. Results: Ninety-six hours after the excimer laser ablation only keratocytes cultured in FBS (Group II) resurface completely. At evaluations after 48, 72 and 96 hours, the residual area in Groups I, III and IV was bigger than in Group II (p < 0.05). There was no significant difference between groups treated with different concentrations of PDGF. Conclusion: Cultured human keratocytes proliferate and migrate to the wounded area after excimer laser ablation and PDGF actually does not stimulate these cells in the presence ofheat-inactivated serum.
| Main Authors: | , , |
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| Format: | Digital revista |
| Language: | English |
| Published: |
Conselho Brasileiro de Oftalmologia
1999
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| Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27491999000200177 |
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