Preservation of protective capacity of hyperimmune anti-Stx2 bovine colostrum against enterohemorrhagic Escherichia coli O157:H7 pathogenicity after pasteurization and spray-drying processes

Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a major etiologic agent that causes bloody diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS). Shiga toxin (Stx) is the main virulence factor of EHEC responsible for the progression to HUS. Although many laboratories have made efforts to develop an effective treatment for Stx-mediated HUS, a specific therapy has not been found yet. Human consumption of bovine colostrum is known to have therapeutic effects against several gastrointestinal infections because of the peptide and proteins (including antibodies) with direct antimicrobial and endotoxin-neutralizing effects contained in this fluid. We have previously demonstrated that colostrum from Stx type 2 (Stx2)-immunized pregnant cows effectively prevents Stx2 cytotoxicity and EHEC O157:H7 pathogenicity. In this study we evaluated the preservation of the protective properties of hyperimmune colostrum against Stx2 (HIC-Stx2) after pasteurization and spray-drying processes by performing in vitro and in vivo assays. Our results showed that reconstituted HIC-Stx2 colostrum after pasteurization at 60°C for 60 min and spray-dried under optimized conditions preserved specific IgG that successfully neutralized Stx2 cytotoxicity on Vero cells. Furthermore, this pasteurized/dehydrated and reconstituted HIC-Stx2 preserved the protective capacity against EHEC infection in a weaned mice model. The consumption of hyperimmune HIC-Stx2 bovine colostrum could be effective for HUS prevention in humans as well as in EHEC control in calves. However, further studies need to be done to consider its use for controlling EHEC infections.

Bibliographic Details

Main Authors:	Garimano, Nicolás, Diaz Vergara, Ladislao Iván, Kim, A. D., Badin, Emiliano Emanuel, Sodero, Sonia, Bernal, Alan Mauro, Gonzalez, Diego, Amaral, María Marta, Lespinard, Alejandro R., Porporatto, Carina, Montenegro, Mariana A., Palermo, Marina Sandra, Larzabal, Mariano, Cataldi, Angel Adrian, Ibarra, Cristina Adriana, Sacerdoti, Flavia
Format:	info:ar-repo/semantics/artículo biblioteca
Language:	eng
Published:	Elsevier 2021-04-28T18:49:32Z
Subjects:	Colostrum, Immune Serum, Bovinae, Pasteurization, Spray Drying, Pathogenicity, Calostro, Suero Inmune, Secado por Pulverización, Escherichia coli, Patogenicidad, Hemolytic Uremic Syndrome, Prevention, Síndrome Urémico Hemolítico, Prevención,
Online Access:	http://hdl.handle.net/20.500.12123/9217 https://www.sciencedirect.com/science/article/abs/pii/S0022030221002678 https://doi.org/10.3168/jds.2020-19709
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