A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolates
Bovine herpesvirus 1 (BoHV-1) causes several clinical syndromes in cattle worldwide. There are 3 subtypes of BoHV-1: 1.1, 1.2a, and 1.2b. Several molecular methods are commonly used in the detection and characterization of BoHV-1. Among them, restriction endonuclease analysis (REA) and single-nucleotide polymorphism (SNP) analysis of the complete viral genome allow classification of BoHV-1 into different subtypes. However, developing countries need simpler and cheaper screening assays for routine testing. We designed a standard multiplex PCR followed by a REA assay allowing straightforward subclassification of all BoHV-1 isolates tested into 1.1, 1.2a, and 1.2b subtypes based on the analysis of fragment length polymorphism. Our standard multiplex PCR-REA was used to analyze 33 field strains of BoHV-1 isolated from various tissues. The assay confirmed the subtype identified previously by REA. In addition, non-polymorphic or undigested fragments were sequenced in order to confirm the mutation affecting the RE HindIII site. Our PCR-REA method is an affordable and rapid test that will subtype all BoHV-1 strains.
| Main Authors: | , , , , |
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| Format: | info:ar-repo/semantics/artículo biblioteca |
| Language: | eng |
| Published: |
Sage Publications
2020-02
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| Subjects: | Bovine Herpesvirus, Genetic Techniques, Genetic Isolation, Herpes Virus Bovino, PCR, Técnica Genética, Aislamiento Genético, BoHV-1, PCR Múltiple, Nuevo Método Molecular, Multiplex PCR, New Molecular Method, |
| Online Access: | http://hdl.handle.net/20.500.12123/7868 https://journals.sagepub.com/doi/abs/10.1177/1040638719898692 https://doi.org/10.1177/1040638719898692 |
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