Microscopic and PCR-based detection of microsporidia spores in human stool samples = Detección microscópica y molecular de esporas de microsporidios en muestras de heces humanas

Microsporidia are obligate intracellular fungi with a remarkable ability to infect a wide range of invertebrate and vertebrate hosts. Namely, Enterocytozoon bieneusi is the most frequently microsporidia reported worldwide, and mainly associated with chronic diarrhea and wasting syndrome in AIDS patients. Microscopy and PCR-based detection techniques are effective for diagnosis and identification of species and genotypes; however, these methods should be standardized in each laboratory. In this study, we performed microscopy and nested PCR techniques with PCR product sequencing to detect E. bieneusi in human stool samples. These techniques, if applied together, might prove useful for diagnosis and future epidemiological studies of intestinal microsporidiosis in Argentina.

Bibliographic Details

Main Authors:	Mena, Cristian Javier, Barnes, Andrés, Castro, Gonzalo, Guasconi, Lorena, Burstein, Verónica Liliana, Beccacece, Ignacio, Paulin, Patricia Catalina, Arneodo Larochette, Joel Demian, Carnevale, Silvana, Astudillo, Germán, Cervi, Laura, Theumer, Martín Gustavo, Chiapello, Laura Silvina
Format:	info:ar-repo/semantics/artículo biblioteca
Language:	eng
Published:	Elsevier 2020-06
Subjects:	Microsporidiosis, Microspora, Infecciones por Protozoos, PCR, Heces, Género Humano, Protozoal Infections, Faeces, Humans, Microsporidios, Microsporidia, Enterocytozoon bieneusi,
Online Access:	http://hdl.handle.net/20.500.12123/7583 https://www.sciencedirect.com/science/article/pii/S0325754120300298 https://doi.org/10.1016/j.ram.2020.04.005
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