An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-Neospora caninum antibodies in cattle
We carried out an inter-laboratory trial to compare the serological tests commonly used for the detection of specific Neospora caninum antibodies in cattle in Ibero-American countries. A total of eight laboratories participated from the following countries: Argentina (n = 4), Brazil (n = 1), Peru (n = 1), Mexico (n = 1), and Spain (n = 1). A blind panel of well-characterized cattle sera (n = 143) and sera representative of the target population (n = 351) was tested by seven in-house indirect fluorescent antibody tests (IFATs 1–7) and three enzyme-linked immunosorbent assays (ELISAs 1–3; two in-house and one commercial). Diagnostic performance of the serological tests was calculated and compared according to the following criteria: (1) the “Pre-test information,” which uses previous epidemiological and serological data; (2) the “Majority of tests,” which classifies a serum as positive or negative according to the results obtained by most tests evaluated. Unexpectedly, six tests showed either sensitivity (Se) or specificity (Sp) values lower than 90%. In contrast, the best tests in terms of Se, Sp, and area under the ROC curve (AUC) values were IFAT 1 and optimized ELISA 1 and ELISA 2. We evaluated a high number of IFATs, which are the most widely used tests in Ibero-America. The significant discordances observed among the tests regardless of the criteria employed hinder control programs and urge the use of a common test or with similar performances to either the optimized IFAT 1 and ELISAs 1 and 2.
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2018-01
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Subjects: | Ganado Bovino, Neospora caninum, Anticuerpos, Experimentación en Laboratorio, Técnicas Inmunológicas, Cattle, Antibodies, Laboratory Experimentation, Immunological Techniques, Ibero América, |
Online Access: | https://link.springer.com/article/10.1007%2Fs11250-017-1401-x http://hdl.handle.net/20.500.12123/2908 https://doi.org/10.1007/s11250-017-1401-x |
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Ganado Bovino Neospora caninum Anticuerpos Experimentación en Laboratorio Técnicas Inmunológicas Cattle Antibodies Laboratory Experimentation Immunological Techniques Ibero América Ganado Bovino Neospora caninum Anticuerpos Experimentación en Laboratorio Técnicas Inmunológicas Cattle Antibodies Laboratory Experimentation Immunological Techniques Ibero América |
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Ganado Bovino Neospora caninum Anticuerpos Experimentación en Laboratorio Técnicas Inmunológicas Cattle Antibodies Laboratory Experimentation Immunological Techniques Ibero América Ganado Bovino Neospora caninum Anticuerpos Experimentación en Laboratorio Técnicas Inmunológicas Cattle Antibodies Laboratory Experimentation Immunological Techniques Ibero América Campero, Lucía María Moreno-Gonzalo, Javier Venturini, María Cecilia Moré, Gastón Dellarupe, Andrea Rambeaud, Magdalena Echaide, Ignacio Eduardo Valentini, Beatriz Susana Campero, Carlos Manuel Moore, Prando Dadin Cano, Dora Beatriz Fort, Marcelo Cristian Mota, Rinaldo A. Serrano-Martínez, Marcos E. Cruz-Vázquez, Carlos Ortega-Mora, Luis M. Álvarez-García, Gema An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-Neospora caninum antibodies in cattle |
description |
We carried out an inter-laboratory trial to compare the serological tests commonly used for the detection of specific Neospora caninum antibodies in cattle in Ibero-American countries. A total of eight laboratories participated from the following countries: Argentina (n = 4), Brazil (n = 1), Peru (n = 1), Mexico (n = 1), and Spain (n = 1). A blind panel of well-characterized cattle sera (n = 143) and sera representative of the target population (n = 351) was tested by seven in-house indirect fluorescent antibody tests (IFATs 1–7) and three enzyme-linked immunosorbent assays (ELISAs 1–3; two in-house and one commercial). Diagnostic performance of the serological tests was calculated and compared according to the following criteria: (1) the “Pre-test information,” which uses previous epidemiological and serological data; (2) the “Majority of tests,” which classifies a serum as positive or negative according to the results obtained by most tests evaluated. Unexpectedly, six tests showed either sensitivity (Se) or specificity (Sp) values lower than 90%. In contrast, the best tests in terms of Se, Sp, and area under the ROC curve (AUC) values were IFAT 1 and optimized ELISA 1 and ELISA 2. We evaluated a high number of IFATs, which are the most widely used tests in Ibero-America. The significant discordances observed among the tests regardless of the criteria employed hinder control programs and urge the use of a common test or with similar performances to either the optimized IFAT 1 and ELISAs 1 and 2. |
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info:ar-repo/semantics/artículo |
topic_facet |
Ganado Bovino Neospora caninum Anticuerpos Experimentación en Laboratorio Técnicas Inmunológicas Cattle Antibodies Laboratory Experimentation Immunological Techniques Ibero América |
author |
Campero, Lucía María Moreno-Gonzalo, Javier Venturini, María Cecilia Moré, Gastón Dellarupe, Andrea Rambeaud, Magdalena Echaide, Ignacio Eduardo Valentini, Beatriz Susana Campero, Carlos Manuel Moore, Prando Dadin Cano, Dora Beatriz Fort, Marcelo Cristian Mota, Rinaldo A. Serrano-Martínez, Marcos E. Cruz-Vázquez, Carlos Ortega-Mora, Luis M. Álvarez-García, Gema |
author_facet |
Campero, Lucía María Moreno-Gonzalo, Javier Venturini, María Cecilia Moré, Gastón Dellarupe, Andrea Rambeaud, Magdalena Echaide, Ignacio Eduardo Valentini, Beatriz Susana Campero, Carlos Manuel Moore, Prando Dadin Cano, Dora Beatriz Fort, Marcelo Cristian Mota, Rinaldo A. Serrano-Martínez, Marcos E. Cruz-Vázquez, Carlos Ortega-Mora, Luis M. Álvarez-García, Gema |
author_sort |
Campero, Lucía María |
title |
An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-Neospora caninum antibodies in cattle |
title_short |
An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-Neospora caninum antibodies in cattle |
title_full |
An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-Neospora caninum antibodies in cattle |
title_fullStr |
An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-Neospora caninum antibodies in cattle |
title_full_unstemmed |
An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-Neospora caninum antibodies in cattle |
title_sort |
ibero-american inter-laboratory trial to evaluate serological tests for the detection of anti-neospora caninum antibodies in cattle |
publishDate |
2018-01 |
url |
https://link.springer.com/article/10.1007%2Fs11250-017-1401-x http://hdl.handle.net/20.500.12123/2908 https://doi.org/10.1007/s11250-017-1401-x |
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oai:localhost:20.500.12123-29082021-04-26T16:49:23Z An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-Neospora caninum antibodies in cattle Campero, Lucía María Moreno-Gonzalo, Javier Venturini, María Cecilia Moré, Gastón Dellarupe, Andrea Rambeaud, Magdalena Echaide, Ignacio Eduardo Valentini, Beatriz Susana Campero, Carlos Manuel Moore, Prando Dadin Cano, Dora Beatriz Fort, Marcelo Cristian Mota, Rinaldo A. Serrano-Martínez, Marcos E. Cruz-Vázquez, Carlos Ortega-Mora, Luis M. Álvarez-García, Gema Ganado Bovino Neospora caninum Anticuerpos Experimentación en Laboratorio Técnicas Inmunológicas Cattle Antibodies Laboratory Experimentation Immunological Techniques Ibero América We carried out an inter-laboratory trial to compare the serological tests commonly used for the detection of specific Neospora caninum antibodies in cattle in Ibero-American countries. A total of eight laboratories participated from the following countries: Argentina (n = 4), Brazil (n = 1), Peru (n = 1), Mexico (n = 1), and Spain (n = 1). A blind panel of well-characterized cattle sera (n = 143) and sera representative of the target population (n = 351) was tested by seven in-house indirect fluorescent antibody tests (IFATs 1–7) and three enzyme-linked immunosorbent assays (ELISAs 1–3; two in-house and one commercial). Diagnostic performance of the serological tests was calculated and compared according to the following criteria: (1) the “Pre-test information,” which uses previous epidemiological and serological data; (2) the “Majority of tests,” which classifies a serum as positive or negative according to the results obtained by most tests evaluated. Unexpectedly, six tests showed either sensitivity (Se) or specificity (Sp) values lower than 90%. In contrast, the best tests in terms of Se, Sp, and area under the ROC curve (AUC) values were IFAT 1 and optimized ELISA 1 and ELISA 2. We evaluated a high number of IFATs, which are the most widely used tests in Ibero-America. The significant discordances observed among the tests regardless of the criteria employed hinder control programs and urge the use of a common test or with similar performances to either the optimized IFAT 1 and ELISAs 1 and 2. EEA Rafaela Fil: Campero, Lucía María. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Moreno-Gonzalo, Javier. Universidad Complutense de Madrid. Facultad de Ciencias Veterinarias. Departamento de Salud Animal. SALUVET; España Fil: Venturini, María C. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina Fil: Moré, Gastón. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Dellarupe, Andrea. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rambeaud, Magdalena. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina Fil: Echaide, Ignacio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Valentini, Beatriz Susana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Moore, Prando Dadin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Cano, Dora Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Fort, Marcelo Cristian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina Fil: Mota, Rinaldo A. Rural Federal University of Pernambuco. Veterinary Medicine Department; Brasil Fil: Serrano-Martínez, Marcos E. Universidad Peruana Cayetano Heredia. Facultad de Medicina Veterinaria y Zootecnica; Perú Fil: Cruz-Vázquez, Carlos. Instituto Técnico El Llano; México Fil: Ortega-Mora, Luis M. Universidad Complutense de Madrid. Facultad de Ciencias Veterinarias. Departamento de Salud Animal. SALUVET; España Fil: Álvarez-García, Gema. Universidad Complutense de Madrid. Facultad de Ciencias Veterinarias. Departamento de Salud Animal. SALUVET; España 2018-07-30T12:01:09Z 2018-07-30T12:01:09Z 2018-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007%2Fs11250-017-1401-x http://hdl.handle.net/20.500.12123/2908 0049-4747 1573-7438 https://doi.org/10.1007/s11250-017-1401-x eng info:eu-repo/semantics/restrictedAccess application/pdf Tropical Animal Health and Production 50 (1) : 75–84 (January 2018) |