An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-Neospora caninum antibodies in cattle

We carried out an inter-laboratory trial to compare the serological tests commonly used for the detection of specific Neospora caninum antibodies in cattle in Ibero-American countries. A total of eight laboratories participated from the following countries: Argentina (n = 4), Brazil (n = 1), Peru (n = 1), Mexico (n = 1), and Spain (n = 1). A blind panel of well-characterized cattle sera (n = 143) and sera representative of the target population (n = 351) was tested by seven in-house indirect fluorescent antibody tests (IFATs 1–7) and three enzyme-linked immunosorbent assays (ELISAs 1–3; two in-house and one commercial). Diagnostic performance of the serological tests was calculated and compared according to the following criteria: (1) the “Pre-test information,” which uses previous epidemiological and serological data; (2) the “Majority of tests,” which classifies a serum as positive or negative according to the results obtained by most tests evaluated. Unexpectedly, six tests showed either sensitivity (Se) or specificity (Sp) values lower than 90%. In contrast, the best tests in terms of Se, Sp, and area under the ROC curve (AUC) values were IFAT 1 and optimized ELISA 1 and ELISA 2. We evaluated a high number of IFATs, which are the most widely used tests in Ibero-America. The significant discordances observed among the tests regardless of the criteria employed hinder control programs and urge the use of a common test or with similar performances to either the optimized IFAT 1 and ELISAs 1 and 2.

Bibliographic Details

Main Authors:	Campero, Lucía María, Moreno-Gonzalo, Javier, Venturini, María Cecilia, Moré, Gastón, Dellarupe, Andrea, Rambeaud, Magdalena, Echaide, Ignacio Eduardo, Valentini, Beatriz Susana, Campero, Carlos Manuel, Moore, Prando Dadin, Cano, Dora Beatriz, Fort, Marcelo Cristian, Mota, Rinaldo A., Serrano-Martínez, Marcos E., Cruz-Vázquez, Carlos, Ortega-Mora, Luis M., Álvarez-García, Gema
Format:	info:ar-repo/semantics/artículo biblioteca
Language:	eng
Published:	2018-01
Subjects:	Ganado Bovino, Neospora caninum, Anticuerpos, Experimentación en Laboratorio, Técnicas Inmunológicas, Cattle, Antibodies, Laboratory Experimentation, Immunological Techniques, Ibero América,
Online Access:	https://link.springer.com/article/10.1007%2Fs11250-017-1401-x http://hdl.handle.net/20.500.12123/2908 https://doi.org/10.1007/s11250-017-1401-x
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